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clontech chroma spintm te  (TaKaRa)


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    Structured Review

    TaKaRa clontech chroma spintm te
    Clontech Chroma Spintm Te, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/clontech+chroma+spintm+te/CHROMA+SPIN+%2BTE-1000+Columns/pm41720382-108-8-7
    Average 94 stars, based on 149 article reviews
    clontech chroma spintm te - by Bioz Stars, 2026-10
    94/100 stars

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    Purification:

    Article Title: Screening and Identification of Host Factors Interacting with the Virulence Factor P0 Encoded by Sugarcane Yellow Leaf Virus by Yeast Two-Hybrid Assay
    Article Snippet: .. The purified double-stranded cDNA was averaged, and then small segments were removed by Clontech CHROMA SPINTM+TE-1000 Columns (Cat. No.: 636079, Takara Biomedical Technology (Beijing) Co., Ltd, Beijing, China). .. The double-stranded cDNA was homologously recombined with the pGADT7 linearized vector, and then the product was shocked into the Escherichia coli TOP10 competent cells and then transferred to Super Optimal broth (SOC) liquid medium and cultured at 37 °C for 1 h. Dilute (1000-fold) 10 µL bacterial solution and 100 µL of bacterial solution were cultured on Luria–Bertani (LB) solid medium (containing 100 µg · mL −1 ampicillin) overnight, and glycerol was added to the remaining bacterial solution (the final concentration was 20%) as library bacterial solution.

    Article Title: Screening and Identification of Host Factors Interacting with the Virulence Factor P0 Encoded by Sugarcane Yellow Leaf Virus by Yeast Two-Hybrid Assay.
    Article Snippet: .. The purified double-stranded cDNA was averaged, and then small segments were removed by Clontech CHROMA SPINTM+TE-1000 Columns (Cat. No.: 636079, Takara Biomedical Technology (Beijing) Co., Ltd, Beijing, China). .. The double-stranded cDNA was homologously recombined with the pGADT7 linearized vector, and then the product was shocked into the Escherichia coli TOP10 competent cells and then transferred to Super Optimal broth (SOC) liquid medium and cultured at 37 ◦C for 1 h. Dilute (1000-fold) 10 µL bacterial solution and 100 µL of bacterial solution were cultured on Luria–Bertani (LB) solid medium (containing 100 µg ·mL−1 ampicillin) overnight, and glycerol was added to the remaining bacterial solution (the final concentration was 20%) as library bacterial solution.

    Homogenization:

    Article Title: Ectopic expression of safflower NAC transcription factor CtNAC78 enhances drought tolerance in Arabidopsis thaliana
    Article Snippet: .. Subsequently, the cDNA homogenization and small fragment removal were performed using Hieff NGS ® Smarter DNA Clean Bead and Clontech CHROMA SPINTM+TE-1000 Columns kit provided by Yeasen Biotechnology Co., Ltd., and TaKaRa. .. After the homogenization treatment and removal of small fragments, the purified cDNA was homologously recombined with a linearized pGADT7 vector and further co-transformed into E. coli (TOP10) by electroshock (Bio-RAD, Inc., USA).

    Next-Generation Sequencing:

    Article Title: Ectopic expression of safflower NAC transcription factor CtNAC78 enhances drought tolerance in Arabidopsis thaliana
    Article Snippet: .. Subsequently, the cDNA homogenization and small fragment removal were performed using Hieff NGS ® Smarter DNA Clean Bead and Clontech CHROMA SPINTM+TE-1000 Columns kit provided by Yeasen Biotechnology Co., Ltd., and TaKaRa. .. After the homogenization treatment and removal of small fragments, the purified cDNA was homologously recombined with a linearized pGADT7 vector and further co-transformed into E. coli (TOP10) by electroshock (Bio-RAD, Inc., USA).



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    94
    TaKaRa clontech chroma spintm te
    Clontech Chroma Spintm Te, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/clontech+chroma+spintm+te/CHROMA+SPIN+%2BTE-1000+Columns/pm41720382-108-8-7
    Average 94 stars, based on 1 article reviews
    clontech chroma spintm te - by Bioz Stars, 2026-10
    94/100 stars
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